Characterization of a Schwann cell enhancer in the myelin basic protein gene

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A distal upstream enhancer from the myelin basic protein gene regulates expression in myelin-forming schwann cells.

In peripheral nerves, large caliber axons are ensheathed by myelin-elaborating Schwann cells. Multiple lines of evidence demonstrate that expression of the genes encoding myelin structural proteins occurs in Schwann cells in response to axonal instructions. To gain further insight into the mechanisms controlling myelin gene expression, we used reporter constructs in transgenic mice to search fo...

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Myelin basic protein gene contains separate enhancers for oligodendrocyte and Schwann cell expression

The DNA sequence between position +36 and -1907 of the murine myelin basic protein gene contains the enhancer and promoter elements necessary for abundant and cell specific expression in transgenic mice. Surprisingly, the pattern of expression promoted by this DNA fragment is a subset of that exhibited by the endogenous myelin basic protein (MBP) gene. Fusion genes prepared with this promoter/e...

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Functional organization of a Schwann cell enhancer.

Myelin basic protein (MBP) gene expression is conferred in oligodendrocytes and Schwann cells by different upstream enhancers. In Schwann cells, expression is controlled by a 422 bp enhancer lying -9 kb from the gene. We show here that it contains 22 mammalian conserved motifs > or =6 bp. To investigate their functional significance, different combinations of wild-type or mutated motifs were in...

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ژورنال

عنوان ژورنال: Journal of Neurochemistry

سال: 2004

ISSN: 0022-3042,1471-4159

DOI: 10.1111/j.1471-4159.2004.02745.x